Routine & Special Histotechnology

Routine Histotechnology

Approximately 30'000 tissues can be trimmed, processed, embedded and stained by HE staining per month.

Histological routine stains and histochemical technologies and methods including PAS, Perls, Oil Red O, are standard.

Many other staining and histotchemical methods available and in use, e.g. Azan, Methylgreen-Pyronin, depending on specific request.

  • Example: Fetal Rabbit Eye, HE

    Example: Fetal Rabbit Eye, HE

  • Example: Rat Liver, PAS

    Example: Rat Liver, PAS

  • Example: Rat Liver, Perl's stain

    Example: Rat Liver, Perl's stain

  • Example: Mouse Liver, Oil Red O

    Example: Mouse Liver, Oil Red O

Special Histology: Bird, Fish, Frog, Avertebrates

- Cutting edge pathology evaluation meets biology
- Several years experience in histopathology evaluation of birds,
different fish species and avertebrates (including snails, bivalves, earthworms and others)

  •  Example: Rainbow trout, Azan

    Example: Rainbow trout, Azan

  • Example: Xenopus, tadpole

    Example: Xenopus, tadpole

  • Example: Snail, Reproductive System, Masson's Trichrom

    Example: Snail, Reproductive System, Masson's Trichrom

  • Example: Earthworm, HE

    Example: Earthworm, HE

Special Histology: Neuropathology Processing

- Perfusion Techniques - on your site
- Plastic Embedding (resin epoxid for semi thin sectioning at 100nm to 300nm)
- Special Staining methods: e.g. Toluidin Blue, Bodian Stains, detection
of degenerated neurons with Fluoro Jade etc.
- Image Analysis
- Under the guidance of highly experienced pathologists

  • Example: Rat: Development Neurotoxicity studies. Measurements of areas, distances, etc.

    Example: Rat: Development Neurotoxicity studies. Measurements of areas, distances, etc.

  • Example: Rat: Hippocampus with degenerated neurons, Fluoro Jade stain

    Example: Rat: Hippocampus with degenerated neurons, Fluoro Jade stain

  • Example: Rat: Excellent perfused brain, hippocampus region, HE

    Example: Rat: Excellent perfused brain, hippocampus region, HE

  • Example: Rat: Normal ganglion: Plastic Embedding, Tolouidin stain

    Example: Rat: Normal ganglion: Plastic Embedding, Tolouidin stain

Special Histology: Plastic/Resin Embedding

Plastic embedding techniques (PMMA or Technovit) for implation studies and non decalcified material (e.g. bones). Sample trimming with diamon saw (EXACT System) and use of sandwich-techniques.

Different staining methods available: e.g. Paragon, Masson's Trichrom, Toluidin Blue, Giemsa, etc.

  • Example: Cutting of embedded block by sandwich technique

    Example: Cutting of embedded block by sandwich technique

  • Example: Result after cutting

    Example: Result after cutting

  • Example: Grinded, polished and stained cut with bone implant (Paragon Stain)

    Example: Grinded, polished and stained cut with bone implant (Paragon Stain)

  • Example: Grinded, polished and stained non-decalcified bone with special stain for osteoclasts, Trap Stain

    Example: Grinded, polished and stained non-decalcified bone with special stain for osteoclasts, Trap Stain

Special Histology: Special Trimming

Techniques for rodents adapted according to Young including longitudinal section of the nasopharyngeal duct (4 transversal section for rat and hamsters, 3 transversal sections for mouse). Standardised technique for dog nasal cavaties (4 transversal sections) and monkey (3 transversal sections) nasal cavaties. Standardised technique for larynx (according to Sagartz) and lower respiratory tract pathology for rodents.

Several publications and presentations as first and co-authors.

  • Example: Rat, levels modified according to Young

    Example: Rat, levels modified according to Young

  • Example: Dog, high quality nasal section, HE stained

    Example: Dog, high quality nasal section, HE stained

  • Example: Dog, standard cutting with diamond band saw

    Example: Dog, standard cutting with diamond band saw

  • Example: Dog, standard levels

    Example: Dog, standard levels

Please contact us for more information: info@anapath.ch

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