Molecular Pathology

- Biomarker
- Immunohistochemistry (ICH)
- Tissue Cross Reactivity (TCR)
- In-Situ Hybridizytion (ISH, FISH)
- Laser Capture Microdissection (LCM)


Biomarker

With over 15 years experience in biomarker for the pre-clinical development our pathologists and toxicologists will support you to answer the critical questions. Which marker can give you insight in your MOA analysis or which marker should integrate in your pre-clinical study in preparation of the clinical phase. We can select from a portfolio of marker and technologies to choose the best approach for you.


Immunohistochemistry

AnaPath is well equipped with a Leica BOND-III system for fully automated IHC. The Leica BOND-III system secures reliable and constant results on fresh frozen or FFPE samples. Based on our experience with numerous established marker we can work out efficiently protocols for your marker and conduct studies under GLP or non-GLP. Qualitative review by a pathologist or quantitative analysis aided by computer assisted tools can provide e.g. absolute or relative areas, particle analysis, structures.


  • Example: Rat liver (treated by pharmaceutical), Ki67

    Example: Rat liver (treated by pharmaceutical), Ki67

  • Example: Particle analysis for counting of nuclei

    Example: Particle analysis for counting of nuclei

TCR-Analysis

GLP TCR can be conducted on human tissue panels following FDA and EMA requirements and on many pre-clinical study species like mice, rat, dog and monkey. All tissue samples are evaluated by a pathologist to guarantee a tissue panel of highest quality. Again the Leica BOND-III system managed by our experienced technicians secures reliable and constant results.


  • Example: CD20 antibody in human bone marrow

    Example: CD20 antibody in human bone marrow

In-Situ Hybridizytion

We offer In-Situ Hybridization (ISH) or Fluorescent In-Situ Hybridization (FISH) by the fully automated Leica Bond III system under GLP. The data will be evaluated by our pathologists.


ATMP's

Including gen therapeutic, somatic cell therapeutics and engineered tissues

- Quality control on bioreactor products
- Qualification of cell lines during GMP production process
- Analysis of engineered tissue samples
- Analysis of in-vivo samples


  • Example: Detection of aggrecan in treated sheep intravertebral disk nucleus pulposus

    Example: Detection of aggrecan in treated sheep intravertebral disk nucleus pulposus

  •  Example: Smooth Muscle Actin-Positivity in cell culture (chamber slides) for quality control on bioreactor cell lines

    Example: Smooth Muscle Actin-Positivity in cell culture (chamber slides) for quality control on bioreactor cell lines

  • Example: Cell count determination on engineered tissue

    Example: Cell count determination on engineered tissue

Laser Capture Microdissection

Laser Capture Microdissection (LCM) is an automated sample preparation technique that enables isolation of tissue regions or specific cells from a mixed population under microscopic visualization.

AnaPath is using the Applied Biosystems® ArcturusXT™ LCM System, which is a unique microdissection instrument that offers the power of two lasers—combining the best of two worlds.

The pathologists at AnaPath apply the gentle solid-state IR laser for dissection and capturing cells.

This technique was designed for optimal biomolecule recovery from an individual target cell or areas smaller than 30 µm in diameter. Using a very low power IR laser to activate a thermoplastic film, causing the film to become sticky and adhere to the cells of interest. When the film is lifted away from the sample preparation, the desired cells are fused to the membrane and remain intact for the recovery of nucleic acids or proteins.

For other applications like the isolation of larger structures or whole tumours the powerful UV laser is the better tool. The UV laser burns efficiently through tissue sections of increased thickness and resilient nature like connective tissue. This approach provides you with a decent amount of tissue for sometimes material consuming downstream processing of nucleic acids or proteins.

At AnaPath we use tissue samples as frozen or FFPE samples for microdissection and can conduct LCM under GLP.

The applications for LCM are as numerous as are the detailed requirements of our clients, hence each service project is bespoken.




Please contact us for more information: info@anapath.ch

News

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24.11.2016

AnaPath welcomes LPT to the Safety Alliance network!

For more infromation please visit:

www.safety-alliance.net .

 

Contact

  AnaPath GmbH AnaPath Services
   Buchsweg 56 Hammerstrasse 49
   4625 Oberbuchsiten 4410 Liestal
   
   0041 62 393 01 61 0041 61 906 40 00
   info@anapath.ch info@anapath.ch